2021 JO Cup Participants - 18U Division
| Team Name | Coach | Location |
|---|---|---|
| Atlanta Vipers Gold 18U Holbrook | Jason Holbrook | Georgia |
| Atlanta Vipers 18U - Shumeyko | Greg Shumeyko | Georgia |
| Carolina Elite Webster | Rick Webster |
North Carolina |
| Chicago Cheetahs | Paul Malito | Chicago |
| Clearwater Bullets - Klinefelter | John Klinefelter | Florida |
| Finesse 18U Dreher/Curry | Donny Dreher | Detroit |
| FL Impact 18U Affrunti/Hoopes | Bill Hoopes/Patrick Affrunti | Florida |
| Florida Select Davis | Rick Davis | Florida |
| Florida Storm Premier Ralph | Ralph Rodriguez | Florida |
| GA Power Gold – Berkhiser/Pisciotta | Jay Worley | Georgia |
| Gold Coast Hurricanes - Cooper/Hammond | Marty Cooper | Florida |
| Hanover Lady Hornets | Chad Radcliffe/Jeff Keys | Virginia |
| Lady Gators Jackson | Randy Jackson | Florida |
| LLG DPS Corn | John Corn | North Carolina |
| Louisiana Blazers Elite | Todd Whittington | Louisiana |
| Miami Stingrays Tampa - Byers 18U | Amanda Byers | Florida |
| Ohio Hawks National 18U | Wendell Hutchinson | Ohio |
| Prospex Gold 18U | Matt Adams | Alabama |
| Stars National Gold - Belfield | Philip Belfield | North Carolina |
| Tampa Mustangs TJ | TJ Goelz | Florida |
| Team NC 18U – Huss | Brad Huss | North Carolina |
| TN Mojo 2021 - Sims | Barry Sims | Georgia |
| Wagner's 3N2 - Haynes | CJ Haynes | Florida |
| Windermere Wildfire 18U – Paulson | George Paulson | Florida |